期刊
MYCOLOGICAL RESEARCH
卷 110, 期 -, 页码 1475-1481出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.mycres.2006.08.009
关键词
biocontrol; dsRNA curing; entomopathogenic fungi; MET; mycoviruses
类别
Beauveria bassiana strains from different hosts and geographic origins were assayed for the presence of double-stranded RNA (dsRNA). Two of them (15.4%) showed extra bands, with approximately 4.0-3.5 kb and 2-0.7 kb, respectively, after electrophoretic separation of undigested nucleic acids. Virus-like particles were approximately 28-30 nm diam. The dsRNA was maintained after conidiogenesis (vertical transmission) and was transmitted horizontally by hyphal anastomosis. Strains purged of dsRNA obtained after cycloheximide treatment showed increased conidial production when compared with strains carrying dsRNA particles. Bioassays demonstrated hypovirulence associated with dsRNA. The mean mortality against the insect Euschistus heros was reduced in strains containing dsRNA when compared with the isogenic dsRNA-free ones. (c) 2006 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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