期刊
PROSTAGLANDINS & OTHER LIPID MEDIATORS
卷 81, 期 3-4, 页码 178-183出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.prostaglandins.2006.09.005
关键词
prostaglandins; osteoblasts; EP2 receptor; EP4 receptor; bone formation
资金
- NIAMS NIH HHS [R01 AR018063-30] Funding Source: Medline
We compared the direct effects of selective EP4 and EP2 receptor agonists (EP4A and EP2A) with prostaglandin E, (PGE,) on the differentiation of cultured murine calvarial osteoblastic cells. EP4A increased alkaline phosphatase activity and osteocalcin mRNA levels in these cultures similar to PGE,. This effect was seen with both direct plating immediately after isolating the cells, or indirect plating in which the cells were grown to confluence and replated. EP2A had a smaller effect, significant only in indirect plating experiments. All three agents decreased the DNA and protein content in indirect plating experiments, but not in direct plating experiments. We conclude that the anabolic effect of PGE, in calvarial osteoblastic cell cultures is largely mediated by activation of the EP4 receptor, while activation of the EP2 receptor is less effective. (c) 2006 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据