期刊
MOLECULAR ECOLOGY NOTES
卷 6, 期 4, 页码 1025-1029出版社
BLACKWELL PUBLISHING
DOI: 10.1111/j.1471-8286.2006.01417.x
关键词
Eubalaena; microsatellite; multiplex PCR; right whale
A North Atlantic right whale (Eubalaena glacialis) genomic library was developed and screened with a (GATA)(8) probe to identify tetranucleotide microsatellite loci. Sixteen characterized loci were polymorphic in North Atlantic and/or South Atlantic (Eubalaena australis) right whales, 12 being polymorphic in E. glacialis, and 15 in E. australis. Fourteen of these were combined with 21 other previously identified loci for a suite of 35 loci which can be used to increase resolution of genetic analyses of these species. Multiplex reactions were developed for genotyping samples at these loci, providing a method that is rapid, reliable and cost-effective.
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