4.5 Article

Trypsin is the primary mechanism by which the 18O isotopic label is lost in quantitative proteomic studies

期刊

ANALYTICAL BIOCHEMISTRY
卷 359, 期 1, 页码 26-34

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2006.08.036

关键词

stable isotope labeling; quantitative proteomics

资金

  1. NCRR NIH HHS [P41RR018502, P41 RR005351, P41 RR018502, P41RR005351, P41 RR005351-16, P41 RR018502-04] Funding Source: Medline

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Labeling with O-18 is currently one of the most commonly used methods for incorporating a stable isotopic label into samples for comparative proteomic studies. In this approach, isotopic labeling involves the enzymatic digestion, typically performed with trypsin, of a protein population in O-18-water, which incorporates the stable isotope into the C termini of the newly formed peptides. Although trypsin is often used to facilitate isotopic incorporation after digestion, it is typically overlooked that this same mechanism can lead to isotopic loss even under conditions such as low pH where it is assumed that trypsin is inactive. To examine the role that trypsin plays in isotopic loss, several experiments were performed on the rate of delabeling under conditions relevant to multidimensional proteomic experiments. Results from these studies demonstrate that enzyme-facilitated exchange of O-18 in the peptide with O-16 in the aqueous solvent was the major process by which the label is removed from the peptides, even under conditions of low pH and temperature where trypsin is thought to be inactive. This study brings the rapid, tryptic-facilitated exchange to the attention of laboratories using this scheme to prevent inaccuracies in quantitative labeling due to loss of the isotopic label. (c) 2006 Elsevier Inc. All rights reserved.

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