期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 26, 期 24, 页码 9387-9401出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.01335-06
关键词
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资金
- Biotechnology and Biological Sciences Research Council [BB/C510291/1] Funding Source: researchfish
- Medical Research Council [G0300662B] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BB/C510291/1] Funding Source: Medline
- NCI NIH HHS [CA100076, R56 CA100076, R01 CA100076] Funding Source: Medline
In eukaryotes, three pairs of structural-maintenance-of-chromosome (SMC) proteins are found in conserved multisubunit protein complexes required for chromosomal organization. Cohesin, the Smc1/3 complex, mediates sister chromatid cohesion while two condensin complexes containing Smc2/4 facilitate chromosome condensation. Smc5/6 scaffolds an essential complex required for homologous recombination repair. We have examined the response of smc6 mutants to the inhibition of DNA replication. We define homologous recombination-dependent and -independent functions for Smc6 during replication inhibition and provide evidence for a Rad60-independent function within S phase, in addition to a Rad60-dependent function following S phase. Both genetic and physical data show that when forks collapse (i.e., are not stabilized by the Cds1(Chk2) checkpoint), Smc6 is required for the effective repair of resulting lesions but not for the recruitment of recombination proteins. We further demonstrate that when the Rad60-dependent, post-S-phase Smc6 function is compromised, the resulting recombination-dependent DNA intermediates that accumulate following release from replication arrest are not recognized by the G(2)/M checkpoint.
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