期刊
MOLECULAR ECOLOGY NOTES
卷 6, 期 4, 页码 959-964出版社
WILEY
DOI: 10.1111/j.1471-8286.2006.01470.x
关键词
COI; DNA barcoding; DNA degradation; fish; Lepidoptera; museum specimens; parasitic wasps; taxonomy
A DNA barcode based on 650 bp of mitochondrial gene cytochrome c oxidase I is proving to be highly functional in species identification for various animal groups. However, DNA degradation complicates the recovery of a full-length barcode from many museum specimens. Here we explore the use of shorter barcode sequences for identification of such specimens. We recovered short sequences - i.e. similar to 100 bp - with a single PCR pass from more than 90% of the specimens in assemblages of moth and wasp museum specimens from which full barcode recovery was only 50%, and the latter were usually less than 8 years old. Short barcodes were effective in identifying specimens, confirming their utility in circumstances where full barcodes are too expensive to obtain and the identification comparisons are within a confined taxonomic group.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据