4.4 Article

Macrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary gland

期刊

DEVELOPMENTAL DYNAMICS
卷 235, 期 12, 页码 3222-3229

出版社

WILEY
DOI: 10.1002/dvdy.20972

关键词

mammary gland; macrophage; multi-photon microscope; collagen; development

资金

  1. NCI NIH HHS [R01 CA094173, P01 CA 100324, R01CA 94173, P30 CA 13330] Funding Source: Medline

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Development of the ductal network in the mammary gland is dependent in part on the presence of macrophages. Here we utilize multi-photon microscopy and second harmonic generation to describe terminal end bud 3-dimensional structure and the organization of the surrounding collagen matrix. We have applied this approach to analyze the effect of macrophage deficiency on terminal end bud structure and collagen organization, using mice homozygous for a null mutation in the colony stimulating factor-1 gene (Csf1(op)/Csf1(op)). Primary terminal end buds have an oblong shape, with long collagen I fibers close to the neck of the terminal end bud and radiating upwards in the direction of growth. Around the terminal end buds, the amount of total collagen I detected by antibody staining was not affected by macrophage deficiency. However the amount of collagen I organized into long fibers, detected by second harmonic generation signal, was reduced in Csf1(op)/Csf1(op) mice. Macrophage deficiency also caused terminal end buds to be rounder and shorter. These studies reveal a role for macrophages in collagen fibrillogenesis and in organization of the structure of terminal end buds.

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