期刊
PLANT JOURNAL
卷 48, 期 6, 页码 974-985出版社
BLACKWELL PUBLISHING
DOI: 10.1111/j.1365-313X.2006.02924.x
关键词
functional genomics; full-length cDNA; dominant phenotype; unknown gene; pale green; Arabidopsis
We have developed a novel gain-of-function system that we have named the FOX hunting system (Full-length cDNA Over-eXpressing gene hunting system). We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. About 10 000 independent full-length Arabidopsis cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Each transgenic Arabidopsis contained on average 2.6 cDNA clones and was monitored under various categories such as morphological changes, fertility and leaf color. We found 1487 possible morphological mutants from 15 547 transformants. When 115 pale green T-1 mutants were analyzed, 59 lines represented the mutant phenotypes in more than 50% of the T-2 progeny. Characterization of two leaf color mutants revealed the significance of this approach. We also document mutants from several categories and their corresponding full-length cDNAs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据