期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 22, 期 12, 页码 1267-1273出版社
SPRINGER
DOI: 10.1007/s11274-006-9171-z
关键词
Flavobacterium; plant-microbe interactions; Proteobacteria; tomato plant; 16S rDNA; rhizosphere
The objective of this study was to characterize the bacterial community composition in the bulk soil, rhizosphere soil and root tissue of the tomato plant (Lycopersicum esculentum Mill). 16S ribosomal DNA (rDNA) from the bacterial community was amplified using PCR, and sequence analysis of 16S rDNA clones was subsequently used for bacterial identification and phylogenetic classification. Phylogenetic analysis of clones (total of 68) from the bulk soil, rhizosphere and root tissues showed that about 50% of the bacteria belonged to the alpha-, beta-, gamma-, and delta-Proteobacteria or Cytophaga-Flavobacterium-Bacteroides (CFB) phyla, with only one high G+C clone identified. A number of diverse bacteria were identified within Proteobacteria, while 87% of the bacteria belonged to the genus Flavobacterium within the CFB phylum, which is a unique finding for tomato plants. Our results will be of interest to those wanting to identify bacteria that can promote plant growth or resistance to diseases.
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