期刊
NATURE BIOTECHNOLOGY
卷 24, 期 12, 页码 1569-1571出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt1264
关键词
-
资金
- NIBIB NIH HHS [EB-000205] Funding Source: Medline
The utility of blue fluorescent protein ( BFP) has been limited by its low quantum yield and rapid photobleaching. A library targeting residues neighboring the chromophore yielded a variant with enhanced quantum yield ( 0.55 versus 0.34), reduced pH sensitivity and a 40-fold increase in photobleaching half-life. This BFP, named Azurite, is well expressed in bacterial and mammalian cells and extends the palette of fluorescent proteins that can be used for imaging.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据