期刊
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
卷 118, 期 6, 页码 1350-1356出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2006.07.040
关键词
T cells; atopic dermatitis; memory T cells; Fel d 1
资金
- MRC [G116/150, MC_U137881017] Funding Source: UKRI
- Medical Research Council [MC_U137881017, G116/150] Funding Source: researchfish
- Medical Research Council [G116/150, MC_U137881017] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
Background: Although substantial evidence suggests that T cells are important in the pathogenesis of atopic dermatitis (AD), little is known of the differentiation status of CD4(+) T cells specific for common environmental allergens. Objective: To determine the frequency, differentiation phenotype, and function of circulating allergen-specific CD4(+) T cells in adult individuals with severe persistent AD and controls. Methods: Using tetrameric complexes of an HLA DRB1*0101 restricted epitope from Fel d 1, the major IgE-reactive component of cat dander, we studied ex vivo and cultured T-cell frequency and phenotype in individuals with AD and healthy controls. Cytokine secretion was measured by ex vivo and cultured IFN-gamma, IL-4, and IL-10 enzyme linked immuno-spot analysis. Results: Ex vivo Fel d 1-specific DRB1*0101-restricted CD4(+) T cells express high levels of CCR7, CD62L, CD27, and CD28 and proportionately low levels of tissue-specific homing receptors and T(H)1 and T(H)2 cytokine production, placing the cells largely within the central memory subgroup. Conclusion: Circulating Fel d 1-specific DRB1*0101-restricted CD4(+) T cells maintain central memory capacity, consistent with a potential to contribute to persisting clinical atopic disease. Clinical implications: Persisting central memory characteristics of allergen-specific CD4(+) T cells in individuals with AD may contribute to chronic disease.
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