期刊
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
卷 291, 期 6, 页码 C1193-C1197出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpcell.00230.2006
关键词
calcium transient; dendrites
资金
- NIDA NIH HHS [P50 DA005010, DA05010] Funding Source: Medline
- NINDS NIH HHS [R01 NS39961] Funding Source: Medline
Mitochondria show extensive movement along neuronal processes, but the mechanisms and function of this movement are not clearly understood. We have used high-resolution confocal microscopy to simultaneously monitor movement of mitochondria and changes in intracellular [Ca2+] ([Ca2+](i)) in rat cortical neurons. A significant percentage (27%) of the total mitochondria in cortical neuronal processes showed movement over distances of > 2 mu M. The average velocity was 0.52 mu m/s. The velocity, direction, and pattern of mitochondrial movement were not affected by transient increases in [Ca2+](i) associated with spontaneous firing of action potentials. Stimulation of Ca2+ transients with forskolin (10 mu M) or bicuculline (10 mu M), or sustained elevations of [Ca2+](i) evoked by glutamate (10 mu M) also had no effect on mitochondrial transit. Neither removal of extracellular Ca2+, depletion of intracellular Ca2+ stores with thapsigargin, or inhibition of synaptic activity with TTX (1 mu M) or a cocktail of CNQX (10 mu M) and MK801 (10 mu M) affected mitochondrial movement. These results indicate that movement of mitochondria along processes is a fundamental activity in neurons that occurs independently of physiological changes in [Ca2+](i) associated with action potential firing, synaptic activity, or release of Ca2+ from intracellular stores.
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