期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 26, 期 23, 页码 8992-9002出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.01311-06
关键词
-
资金
- NIGMS NIH HHS [GM 58859, R01 GM058859] Funding Source: Medline
Information must be shared and functions coordinated among the spatially distinct functional centers of the ribosome. To address these issues, a yeast-based genetic system enabling generation of stable strains expressing only mutant forms of rRNA was devised. The B1a bridge (helix 38) has been implicated in the subtle modulation of numerous ribosomal functions. Base-specific mutations were introduced into helix 38 at sites affecting the B1a bridge and where it contacts the aminoacyl-tRNA (aa-tRNA) D-loop. Both sets of mutants promoted increased affinities for aa-tRNA but had different effects in their responses to two A-site-specific drugs and on suppression nonsense codons. Structural analyses revealed an arc of nucleotides in 25S rRNA that link the B1a bridge, the peptidyltransferase center, the GTPase-associated center, and the sarcin/ricin loop. We propose that a series of regularly spaced hinge bases provide fulcrums around which rigid helices can reorient themselves depending on the occupancy status of the A-site.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据