期刊
CURRENT OPINION IN STRUCTURAL BIOLOGY
卷 16, 期 6, 页码 714-721出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2006.10.001
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资金
- NIGMS NIH HHS [R01 GM042618] Funding Source: Medline
It has long been appreciated that green fluorescent protein (GFP) autocatalytically forms its chromophore in a host-independent process; several of the initial steps in the reaction have recently been elucidated. Nevertheless, the end points of the process are unexpectedly diverse, as six chemically distinct chromophores, including two with three rings, have been identified. All fluorescent proteins continuously produce a low level of reactive oxygen species under illumination, which, in some cases, can lead to host cell death. In one extreme but useful example, the red fluorescent protein Killer-Red can be used to selectively destroy cells upon brief illumination. Finally, when photophysical processes such as excited-state proton transfer, reversible photobleaching and photoactivation are understood, useful research tools, for example, real-time biosensors and optical highlighters, can result; however, side effects of their use may lead to significant artifacts in timedependent microscopy experiments.
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