期刊
EMBO REPORTS
卷 7, 期 12, 页码 1228-1232出版社
WILEY
DOI: 10.1038/sj.embor.7400833
关键词
donor-strand exchange; PapH; pili
资金
- Medical Research Council [G0100442] Funding Source: researchfish
- MRC [G0100442] Funding Source: UKRI
- Medical Research Council [G0100442] Funding Source: Medline
P pili are important adhesive fibres that are assembled by the conserved chaperone-usher pathway. During pilus assembly, the subunits are incorporated into the growing fibre by the donor-strand exchange mechanism, whereby the beta-strand of the chaperone, which complements the incomplete immunoglobulin fold of each subunit, is displaced by the amino-terminal extension of an incoming subunit in a zip-in-zip-out exchange process that is initiated at the P5 pocket, an exposed hydrophobic pocket in the groove of the subunit. In vivo, termination of P pilus growth requires a specialized subunit, PapH. Here, we show that PapH is incorporated at the base of the growing pilus, where it is unable to undergo donor-strand exchange. This inability is not due to a stronger PapD-PapH interaction, but to a lack of a P5 initiator pocket in the PapH structure, suggesting that PapH terminates pilus growth because it is lacking the initiation point by which donor-strand exchange proceeds.
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