Flavobacterium glaciei sp nov., a novel psychrophilic bacterium isolated from the China No.1 glacier

A novel psychrophilic, yellow-pigmented and obligate aerobic bacterium, strain 0499(T), was isolated from the China No. 1 glacier. Strain 0499(T) displayed the common phenotypic and chemotaxonomic features of the genus Flavobacterium, containing menaquinone-6 (MK-6) as the major quinone and C-15:0, iso-C-15:0, C(17:1)w6c and summed feature 3 (C(16.1)w7c/iso-C-15:0 2-OH) as the major fatty acids. Optimal growth occurred at 21 degrees C. The genomic DNA G + C content was 36(.)5 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain 0499(T) was related to members of the genus Flavobacterium, sharing the highest sequence similarities with Flavobacterium succinicans (97(.)9%), Flavobacterium granuli (97(.)4%) and Flavobacterium hydatis (97(.)2%). On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, a novel species Flavobacterium glaciei is proposed with strain 0499(T) (=CGMCC 1.5380(T) = JCM 13953(T)) as the type strain.