期刊
JOURNAL OF VETERINARY MEDICAL SCIENCE
卷 68, 期 12, 页码 1335-1338出版社
JAPAN SOC VET SCI
DOI: 10.1292/jvms.68.1335
关键词
genomic diversity; Okinawa; Theileria
Benign Theileria parasites of cattle distributed in the Okinawa prefecture were characterized by allele-specific polymerase chain reaction (PCR) and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. Using universal or allele-specific primer sets, parasite DNA was amplified in 31 out of 48 blood samples obtained from beef cattle. Among the positive cases, mixed infections involving various combinations of I-, C-, and B-type parasites were detected in 24 (77.4%) samples. Phylogenetic analysis based on the MPSP gene sequences revealed that parasites with the MPSP types 1-5 and 7, exist within the Okinawa prefecture.
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