期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 103, 期 50, 页码 18963-18968出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0606146103
关键词
genetic engineering; Helitothis virescens; mosquitoes; plants; digestion
资金
- NIAID NIH HHS [AI 41254] Funding Source: Medline
- NIGMS NIH HHS [GM 37537, R01 GM037537] Funding Source: Medline
We report the engineering of the surface of the tobacco mosaic virus (TMV) virion with a mosquito decapepticle hormone, trypsinmodulating oostatic factor (TMOF). The TMV coat protein (CP) was fused to TMOF at the C terminus by using a read-through, leaky stop codon that facilitated expression of CP and chimeric CP-TMOF (20:1 ratio) that were coassembled into virus particles in infected Nicotiana tabacum. Plants that were infected with the hybrid TMV RNA accumulated TMOF to levels of 1.3% of total soluble protein. Infected tobacco leaf discs that were fed to Heliothis virescens fourth-instar larvae stunted their growth and inhibited trypsin and chymotrypsin activity in their midgut. Purified CP-TMOF virions fed to mosquito larvae stopped larval growth and caused death. Because TMV has a wide host range, expressing TMV-TMOF in plants can be used as a general method to protect them against agricultural insect pests and to control vector mosquitoes.
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