期刊
EMBO JOURNAL
卷 25, 期 24, 页码 5670-5682出版社
WILEY
DOI: 10.1038/sj.emboj.7601459
关键词
anchoring; BICD; centrosome; GSK-3; microtubule
Microtubule arrays direct intracellular organization and define cellular polarity. Here, we show a novel function of glycogen synthase kinase-3b (GSK-3b) in the organization of microtubule arrays through the interaction with Bicaudal-D (BICD). BICD is known to form a complex with dynein-dynactin and to function in the intracellular vesicle trafficking. Our data revealed that GSK-3b is required for the binding of BICD to dynein but not to dynactin. Knockdown of GSK-3b or BICD reduced centrosomally focused microtubules and induced the mislocalization of centrosomal proteins. The unfocused microtubules in GSK-3b knockdown cells were rescued by the expression of the dynein intermediate chain-BICD fusion protein. Microtubule regrowth assays showed that GSK-3b and BICD are required for the anchoring of microtubules to the centrosome. These results imply that GSK-3b may function in transporting centrosomal proteins to the centrosome by stabilizing the BICD1 and dynein complex, resulting in the regulation of a focused microtubule organization.
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