期刊
DEVELOPMENTAL BIOLOGY
卷 300, 期 2, 页码 758-769出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2006.08.062
关键词
Klf7; Bm3a; TrkA; TrkB; TrkC; neurotrophin; sensory neuron; transcription; apoptosis
资金
- NINDS NIH HHS [R37 NS033199] Funding Source: Medline
The zinc finger protein Klf7P and POU homeodomain protein Brn3a are each required for efficient transcription of TrkA in primary sensory neurons. In this study, we examined whether these transcription factors act in concert to regulate TrkA expression. In vitro, Brn3a and Klf7 can synergistically activate the TrkA enhancer. In vivo, precursor cells that are destined to become TrkA(+) neurons are born. However, both Brn3a and Klf7 are dispensable for the initiation of TrkA expression. At El 2.5, while TrkA expression is unaffected in Brn3a-/- trigeminal ganglia and only slightly decreased in Klf7-/- trigeminal ganglia, it is severely reduced in the double mutant Brn3a-/-;Klf7-/- trigeminal ganglia. At birth, all Trk(+) neurons are lost in Brn3a-/-;Klf7-/- trigeminal ganglia. We further demonstrate that the TrkA enhancer is inactive in Brn3a-/-;Klf7-/- trigeminal ganglia. Thus, cooperation between these two transcription factors is required for endogenous TrkA gene expression and the survival of nociceptive sensory neurons. (c) 2006 Elsevier Inc. All rights reserved.
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