期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 79A, 期 4, 页码 1023-1032出版社
WILEY
DOI: 10.1002/jbm.a.31040
关键词
surface analysis; microrough titanium surface; cell number; alkaline phosphatase; Runx2 gene expression; type I collagen
The purpose of this study vas to define the surface properties of prepared titanium (Ti) disks, which served as a model system, and to contrast the biologic response of MG63 cells exposed to Ti disks with different levels of surface roughness. The surface properties interact with each other, resulting in a change of other surface qualities in addition to roughness due to the surface roughening procedure. The machined Ti disks were roughened by sandblasting and electric glow discharging. The surface properties of the Ti specimens were inspected through a comprehensive surface analysis. MG63 cell behaviors were compared along with cell number, alkaline phosphatase (ALP) activity, Runx2 gene expression, and type I collagen production. Statistics were evaluated, using analysis of variance (ANOVA). The sandblasted Ti disks demonstrated well-controlled surface roughness features and meaningful average roughness ranges, including the surface roughness of the modem' microrough implant, used clinically. With increasing Ti surface roughness, the cell number decreased, while the ALP activity, type I collagen production, and Runx2 gene expression increased significantly. The rougher the Ti surface was, the sooner the Runx2 gene was expressed. Based on these results, we suggest that the microrough Ti surfaces of the 1-3 mu m range may contribute effectively to osteogenic differentiation and proliferation in MG63 cells. (c) 2006 Wiley Periodicals, Inc.
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