Sonoporation, anti-cancer drug and antibody delivery using ultrasound

It has been shown experimentally in cell suspensions that sonoporation could be used to deliver the anti- cancer drug Adriamycin hydrochloride ( ADR) into Jurkat lymphocytes and that it could also be used to deliver goat anti- rabbit IgG and anti- mouse IgD, antibodies into human peripheral blood mononuclear cells ( PBMC) and Jurkat lymphocytes. When ADR was delivered, the delivery efficiency reached 4.80 +/- 2.04% and control baseline ( no ultrasound and no ADR) was 0.17 +/- 0.14%. When anti- rabbit IgD was delivered, the efficiencies were 34.90 +/- 1.80% ( control baseline was 1.33 +/- 0.31%) and 32.50 +/- 4.36% ( control baseline was 1.66 +/- 0.06%) respectively for Jurkat cells and PBMC. When goat anti- rabbit IgG was delivered, the efficiencies were 78.60 +/- 3.60% (control baseline was 1.60 +/- 0.00%) and 57.50 +/- 4.23% ( control baseline was 11.30 +/- 0.81%) respectively for Jurkat cells and PBMC. (c) 2006 Elsevier B. V. All rights reserved.