Pseudomonas aeruginosa quorum sensing molecule N-(3 oxododecanoyl)-L-homoserine lactone disrupts epithelial barrier integrity of Caco-2 cells

Acyl-homoserine lactone (HSL) quorum sensing molecules play an important role in regulation of virulence gene expression in Pseudomonas aeruginosa. Here, we show that 30-C-12-HSL can disrupt barrier integrity in human epithelial Caco-2 cells as evidenced by decreased transepithelial electrical resistance (TER), increased paracellular flux, reduction in the expression and distribution of ZO-1 and occludin, and reorganization of F-actin. P. aeruginosa 30-C-12-HSL activate p38 and p42/44 kinases, and inhibition of these kinases partly prevented 30-C-12-HSL-induced changes in TER, paracellular flux and expression of occludin and ZO-1. These findings demonstrate that P. aeruginosa 30-C-12-HSL can modulate tight junction integrity of Caco-2 cells. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.