期刊
ANIMAL SCIENCE JOURNAL
卷 86, 期 4, 页码 378-384出版社
WILEY
DOI: 10.1111/asj.12307
关键词
nitrate reduction; nitrite reduction; rumen microbes; ruminant; Selenomonas ruminantium
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT) [23580377]
- Grants-in-Aid for Scientific Research [26450104, 23580377] Funding Source: KAKEN
This study investigated the effects of dietary nitrate addition on ruminal fermentation characteristics and microbial populations in goats. The involvement of Selenomonas ruminantium in nitrate and nitrite reduction in the rumen was also examined. As the result of nitrate feeding, the total concentration of ruminal volatile fatty acids decreased, whereas the acetate:propionate ratio and the concentrations of ammonia and lactate increased. Populations of methanogens, protozoa and fungi, as estimated by real-time PCR, were greatly decreased as a result of nitrate inclusion in the diet. There was modest or little impact of nitrate on the populations of prevailing species or genus of bacteria in the rumen, whereas Streptococcus bovis and S.ruminantium significantly increased. Both the activities of nitrate reductase (NaR) and nitrite reductase (NiR) per total mass of ruminal bacteria were increased by nitrate feeding. Quantification of the genes encoding NaR and NiR by real-time PCR with primers specific for S.ruminantium showed that these genes were increased by feeding nitrate, suggesting that the growth of nitrate- and nitrite-reducing S.ruminantium is stimulated by nitrate addition. Thus, S.ruminantium is likely to play a major role in nitrate and nitrite reduction in the rumen.
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