Terribacillus saccharophilus gen. nov., sp nov and Terribacillus halophilus sp nov., spore-forming bacteria isolated from field soil in Japan

Three strains, 002-048(T), RB589 and 002-051(T), isolated from field soil in Japan, were characterized using a polyphasic approach. The isolates were Gram-positive, strictly aerobic, non-motile rods that formed ellipsoidal, subterminal endospores. The chemotaxonomic characteristics of these isolates included the presence of meso-diaminopimelic acid as the cell-wall peptidoglycan, anteiso-C-15:0 and anteiso-C-17:0 as the major cellular fatty acids and MK-7 as the predominant menaquinone. The DNA G + C content was 44-46 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the isolates represented an independent lineage that is distinct from related taxa and exhibited less than 94.3% sequence similarity with respect to those taxa. Moreover, a DNA-DNA hybridization analysis showed that the three isolates represented two species. On the basis of their phenotypic and phylogenetic distinctiveness, the isolates represent two species within a novel genus, for which the names Terribacillus saccharophilus gen. nov., sp. nov. and Terribacillus halophilus sp. nov. are proposed. The type strain of T saccharophilus is 002-048(T) (= IAM 15309(T) = KCTC 13936(T)) and the type strain of T halophilus is 002-051(T) (= IAM 15310(T) = KCTC 13937(T)).