期刊
ANIMAL GENETICS
卷 43, 期 5, 页码 611-613出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2052.2012.02328.x
关键词
cattle; haplogroup classification; phylogeny; Y chromosome; Y-specific biallelic markers
资金
- Fondazione Alma Mater Ticinensis
A new sequence-tagged site (STS) was identified within intron 26 of the bovine USP9Y gene, showing an 81-base pair insertion (g.76439_76440ins81 in sequence with GenBank accession FJ195366) able to distinguish Y2 and Y3 Bos Y haplogroups from Y1. Moreover, four Y3-specific sequence variants allow a distinction from haplogroup Y2. The typing of a Bison bison Y chromosome indicates that the ancestral allele for the USP9Y 81-bp insertion is the short Y1 version. The results from typing the new STS in 1230 cattle Y chromosomes are fully consistent with their classification through standard methods. Thanks to the newly identified STS, it is now possible to assign cattle Y chromosomes to the currently known haplogroups using a single marker.
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