期刊
ANIMAL GENETICS
卷 41, 期 5, 页码 449-453出版社
WILEY
DOI: 10.1111/j.1365-2052.2010.02100.x
关键词
genome; Ovis aries; reference; sequence; sheep
资金
- Roslin Institute
- University of Edinburgh
- Biotechnology and Biological Sciences Research Council, UK
- Scottish Government, UK
- Defra/HEFC/SHEFC Veterinary Training and Research Initiative, UK
- USDA-ARS, USA
- USDA-NRICGP, USA [2008-03923, 2009-03305]
- USDA-NRSP-8, USA
- Meat and Livestock Australia and Australian Wool Innovation Limited
- Australian Government, Australia [CG090143]
- Australia
- University of Sydney, Australia
- CSIRO, Australia
- AgResearch, NZ, Meat and Wool NZ through Ovita, NZ
- INRA
- ANR
- European Union
- 973 Program [2007CB815700]
- Chinese Academy of Sciences
- CAS-Max Planck Society
- National Natural Science Foundation of China [30725008]
- Shenzhen [ZYC200903240077A, ZYC200903240078A]
- Danish Natural Science Research Council
- Solexa [272-07-0196]
- Biotechnology and Biological Sciences Research Council [BBS/E/D/05191130] Funding Source: researchfish
- BBSRC [BBS/E/D/05191130] Funding Source: UKRI
P>Until recently, the construction of a reference genome was performed using Sanger sequencing alone. The emergence of next-generation sequencing platforms now means reference genomes may incorporate sequence data generated from a range of sequencing platforms, each of which have different read length, systematic biases and mate-pair characteristics. The objective of this review is to inform the mammalian genomics community about the experimental strategy being pursued by the International Sheep Genomics Consortium (ISGC) to construct the draft reference genome of sheep (Ovis aries). Component activities such as data generation, sequence assembly and annotation are described, along with information concerning the key researchers performing the work. This aims to foster future participation from across the research community through the coordinated activities of the consortium. The review also serves as a 'marker paper' by providing information concerning the pre-publication release of the reference genome. This ensures the ISGC adheres to the framework for data sharing established at the recent Toronto International Data Release Workshop and provides guidelines for data users.
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