期刊
ANIMAL GENETICS
卷 41, 期 6, 页码 570-578出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2052.2010.02046.x
关键词
bovine SNP50; cattle; fertility; genome-wide association; selective DNA pooling
资金
- USDA Hatch [WIS-142-PRJ16JH]
- University of Wisconsin-Madison [MSN132747]
The decline in the reproductive efficiency of dairy cows, especially those with high producing potential, has become a challenging problem. In this study, a selective DNA pooling approach was applied to a cow population whose oocytes were fertilized and cultured to obtain phenotypic records of fertilization rate and blastocyst rate. Using a stringent 5% genome-wide significance level, 22 and five single nucleotide polymorphisms (SNPs) were found to be associated with fertilization rate and blastocyst rate, respectively. SNPs that showed significant association in selective DNA pooling were further evaluated by individual genotyping. Interestingly, the majority of the SNP associations were confirmed by individual genotyping, testifying to the effectiveness of selective DNA pooling using a high-density SNP genotyping array. This study is the first application of the selective DNA pooling approach using the BovineSNP50 array in cattle.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据