期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 13, 期 2, 页码 407-416出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200600719
关键词
helical structures; NMR spectroscopy; peptides; solvent effects; structure elucidation
The two peptides, rich in C-alpha-tetrasubstituted amino acids, Ac-[Aib-L-(alpha Me)Val-Aib](2)-L-His-NH2 (1) and Ac-[Aib-L-(alpha Me)Val-Aib](2)-O-tBu (2a) are prevalently helical. They present the unique property of changing their conformation from the alpha- to the 3(10)-helix as a function of the polarity of the solvent: alpha in more polar solvents: 3(10) in less polar ones. Conclusive evidence of this reversible change of con-formation is reported on the basis of the circular dichroism (CD) spectra and a detailed two-dimensional NMR analysis in two solvents (trifluoroethanol and methanol) refined with molecular dynamics calculations. The X-ray diffractometric analysis of the crystals of both peptides reveals that they assume a prevalent 3(10)-helix conformation. in the solid state. This conformation is practically superimposable on that obtained from the NMR analysis of I in methanol. The NMR results further validate the reported CD signature of the 3(10)-helix and the use of the CD technique for its assessment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据