期刊
FUNCTIONAL & INTEGRATIVE GENOMICS
卷 7, 期 1, 页码 69-77出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10142-006-0028-1
关键词
Fusarium head blight; microarray; gene expression; wheat scab
资金
- NCRR NIH HHS [2P20RR016478-04, 1P20RR16478-02, 5P20RR15564-03] Funding Source: Medline
Fusarium head blight (FHB), primarily caused by Fusarium graminearum Schw., is a. destructive disease of wheat (Triticum,aestivum L.). Although several genes related to FHB resistance have been reported, global analysis of gene expression in response to FHB infection remains to be explored. The expression patterns of transcriptomes from wheat spikes of FHB-resistant cultivar Ning 7840 and susceptible cultivar Clark were monitored during a period,of 72 h after inoculation (hai) with F graminearum. Microarray analysis, coupled with suppression subtractive hybridization technique, identified 44 significantly differentially expressed genes between cv. Ning 7840 and cv. Clark. More differentially expressed genes were identified from susceptible libraries than from resistance libraries. The up-regulation of defense-related genes in Ning 7840 relative to cultivar Clark occurred during early fungal stress (3-12 hai). Three genes, with unknown function that were up-regulated in cv. Ning 7840 at most time points investigated, might play an important role in enhancing FHB resistance.
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