期刊
NEUROPHARMACOLOGY
卷 52, 期 1, 页码 55-59出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuropharm.2006.06.020
关键词
hippocampal slice; adult; ultrastructure; dendritic spine
资金
- NIMH NIH HHS [K01MH02000, K01 MH002000] Funding Source: Medline
- NINDS NIH HHS [NS21184, 1T32NS045543] Funding Source: Medline
The hippocampal slice is a popular model system in which to study the cellular properties of long-term potentiation (UP). Synaptogenesis induced by exposure to ice-cold artificial cerebrospinal fluid (ACSF), however, raises the concern that morphological correlates of UP might be obscured, especially in mature slices. Here we demonstrate that preparation of mature hippocampal slices at room temperature ( similar to 25 degrees C) maintains excellent ultrastructure and a synapse density comparable to perfusion-fixed hippocampus. These results suggest that slices prepared at room temperature might provide a better basis from which to detect LTP-related changes in synapse number and morphology. (c) 2006 Elsevier Ltd. All rights reserved.
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