期刊
ANIMAL FEED SCIENCE AND TECHNOLOGY
卷 155, 期 1, 页码 1-8出版社
ELSEVIER
DOI: 10.1016/j.anifeedsci.2009.09.015
关键词
Winter sika deer; Rumen microbes; Tannic acid; Ca-alginate gel beads; Streptococcus macedonicus
资金
- Japan Society for the Promotion of Science [16208032, 20248033]
- Grants-in-Aid for Scientific Research [20248033, 16208032] Funding Source: KAKEN
In winter seasons, wild sika deer (Cervus nippon yesoensis) inhabiting the Shiretoko Peninsula of Hokkaido Island,japan, mainly graze woody materials (bark and twigs, etc.) as their feed source. Most of the tree species that they feed upon contain a high level of hydrolysable tannins within the inner bark. Tannins generally lead to low protein digestion and nutrient loss to these herbivorous mammals due to tannization of proteins. In winter months, it is speculated that wild sika deer develop a mechanism to degrade the tannins which are contained in their feed sources, but rumen fluid obtained from sika deer ill winter months did not exhibit any ability to degrade tannins in liquid culture medium. However, constant degradation of hydrolysable tannin was observed when Ca-alginate gel beads were used for microbial immobilization and culturing. The gel beads that had been impregnated with 0.6x-10(4) fold-diluted rumen fluid of sika deer in winter and pre-incubated for 24h under anaerobic conditions supplemented with a 1.5 g/L sugar were reacted with 5 g/L tannic acid solution. Under these conditions, the immobilized rumen bacteria grown in the macrogel beads effectively hydrolyzed tannic acid to release gallic acid monomers. Major bacterial colonies emerging in the Ca-alginate gel beads were identified as Streptococcus macedonicus and this bacterium (EC-D140) was regarded as the most likely candidate as the tannin-degrading bacterium. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据