期刊
FOOD CHEMISTRY
卷 105, 期 2, 页码 691-699出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2006.12.063
关键词
profiling; quantification; quercetin glucosides; onion; capillary electrophoresis; high performance liquid chromatography
There is increasing evidence that flavonols demonstrate beneficial properties for human health. Quercetin is the major flavonol present in onion (Allium cepa cu) and is present predominantly as quercetin 3,4'-diglucoside and quercetin 4'-monoglucoside. These compounds are known to be potent free radical scavengers and antioxidants, and are considered to be protective against cardiovascular disease. Analysis for the presence of these compounds has therefore become more important. Robust capillary zone electrophoresis and high performance liquid chromatography procedures were developed for profiling and quantifying the levels of quercetin 3,4'-diglucoside and quercetin 4'-monoglucoside in 70% methanol/water extracts of six different onion varieties available in Victoria, Australia. Quercetin 3,4'-diglucoside, which is not commercially available as a reference standard, was isolated from freeze-dried onion powder by preparative high performance liquid chromatography and used to quantify the levels in the onion extracts. Significant differences in the levels and ratios of the two compounds were seen between red, brown and white onion varieties (e.g. 'Redwing'; quercetin 3,4'-diglucoside 191 mg/100 g DW, quercetin 4'-monoglucoside 85 mg/100 g DW; 'Cream Gold', quercetin 3,4'-diglucoside 153 mg/100 g DW, quercetin 4'-monoglucoside 58 mg/100 g DW, 'Spanish white'; quercetin 3,4'-diglueoside < 1 mg/100 g DW, quercetin 4'-monoglucoside < 1 mg,/100 g DW). Crown Copyright 9 2007 Published by Elsevier Ltd. All rights reserved.
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