期刊
ANIMAL BIOTECHNOLOGY
卷 23, 期 2, 页码 124-131出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10495398.2011.652326
关键词
IL-6; IPEC-J2 cells; Molecular cloning; Porcine Akirin2
资金
- Program for Changjiang Scholars and Innovative Research Team in University [IRTO555-5]
- Sichuan Provincial Education Department [00924700, 00924800]
- Sichuan Agricultural University
Akirin2 is a recently discovered gene that is involved in innate immune response. In this study, the porcine Akirin2 gene was cloned. The full-length coding sequence (CDS) of porcine Akirin2 consists of 612 bp and encodes 203 amino acids with a molecular mass of 22493 kD. The homology tree analysis showed that the pig Akirin2 has closer genetic relationships and distance with the known mammalian Akirin2. Real time quantitative PCR analysis showed that the porcine Akirin2 transcript was most abundant in the lung, followed by the skeletal muscle, heart, liver, fat, thymus, lymph node, small intestine, kidney, and spleen. Overexpression of porcine Akirin2 increased expression of IL-6 in porcine jejunal epithelial cell line IPEC-J2 cells. Our data suggest that porcine Akirin2 could play an important role in intestinal immune regulation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据