期刊
MARINE BIOTECHNOLOGY
卷 9, 期 1, 页码 82-91出版社
SPRINGER
DOI: 10.1007/s10126-006-6050-1
关键词
cell transplantation; chimera; embryonic stem cells; pluripotency; sea perch; zebrafish
A stable GFP-expressing (GFP(+)LJES1) cell strain was developed from the LJES1 cells obtained from sea perch (Lateolabrax japonicus,) embryos. GFP(+)LJES1 cells were induced in vitro by RA to differentiate into a variety of cell types and also had the ability to form embryoid body-like structures in suspension culture. To determine the differentiation potential of LJES1 cells in vivo, GFP(+)LJES1 cells were transplanted into sea perch and zebrafish embryos at mid-blastula stage. Twenty out of 478 transplanted sea perch embryos contained GFP-expressing LJES1 cells 24 h after microinjection. Fifteen chimera embryos developed into fry. In these chimeras, the GFP(+)LJES1 cells contributed to a variety of tissues including the head and trunk. In zebrafish, 221 embryos were microinjected with GFP(+)LJES1 cells and 22 chimera embryos and fries expressing GFP were obtained. Donor GFP(+)LJES1 cells contributed to various tissues in head and trunk of zebrafish embryos and hatched fry.
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