期刊
BRAIN RESEARCH
卷 1127, 期 1, 页码 36-44出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2006.09.110
关键词
locus coeruleus; cognition; electron microscopy; confocal microscopy
资金
- NIDA NIH HHS [K02 DA015395, DA 020129, DA15395, DA09082, R01 DA020129, R01 DA009082] Funding Source: Medline
Cannabinoid agonists exert complex actions on modulatory neurotransmitters involved in attention and cognition. Previous studies have demonstrated that acute systemic administration of the synthetic cannabinoid agonist, WIN 55,212-2, increases norepinephrine efflux in the rat frontal cortex. In an effort to elucidate whether cannabinoid (CB1) receptors are positioned to presynaptically modulate norepinephrine release in the frontal cortex, immunocytochemical detection of the CB1 receptor and the catecholamine-synthesizing enzyme dopamine-beta-hydroxylase (D beta H) was performed using confocal immunofluorescence microscopy and immunoelectron microscopy in rat brain. Fluorescence microscopy analysis of dually labeled tissue sections from the frontal cortex indicated that individual axonal processes exhibited both CB1 receptor and D H immunoreactivities. Ultrastructural analysis confirmed that one-third of axon terminals containing CB1 immunolabeling also exhibited D beta H labeling. Cortical neurons were also found to be targeted by separately labeled CB1- and D beta H-containing axon terminals. In conclusion, the present neuroanatomical data suggest that cortical norepinephrine release may be modulated, in part, by CB1 receptors that are presynaptically distributed on noradrenergic axon terminals. (c) 2006 Elsevier B.V. All rights reserved.
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