期刊
JOURNAL OF NEUROSCIENCE
卷 27, 期 2, 页码 341-354出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4051-06.2007
关键词
synaptic vesicle recycling; hippocampal slice; short term synaptic depression; vesicle reacidification; endocytosis; CA1; Schaffer collateral
资金
- NIMH NIH HHS [MH068437, R01 MH068437] Funding Source: Medline
During short-term synaptic depression, neurotransmission rapidly decreases in response to repetitive action potential firing. Here, by blocking the vacuolar ATPase, alkalinizing the extracellular pH, or exposing hippocampal slices to pH buffers, we impaired neurotransmitter refilling, and electrophysiologically tested the role of vesicle reuse in synaptic depression. Under all conditions, synapses onto hippocampal CA1 pyramidal cells showed faster depression with increasing stimulation frequencies. At 20 Hz, compromising neurotransmitter refilling increased depression within 300 ms reaching completion within 2 s, suggesting a minimal contribution of reserve vesicles to neurotransmission. In contrast, at 1 Hz, depression emerged gradually and became significant within 100 s. Moreover, the depression induced by pH buffers was reversible with a similar frequency dependence, suggesting that the frequency-dependent increase in depression was caused by impairment of rapid synaptic vesicle reuse. These results indicate that synaptic vesicle trafficking impacts the kinetics of short-term synaptic plasticity at an extremely rapid time scale.
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