期刊
SCIENCE
卷 315, 期 5809, 页码 253-255出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1136264
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Environmental sequencing has revealed unimagined diversity among eukaryotic picoplankton. A distinct picoplanktonic algal group, initially detected from 18S ribosomal DNA ( rDNA) sequences, was hybridized with rRNA lambda-targeted (rRNA-targeted) probes, detected by tyramide signal amplification-fluorescent in situ hybridization, and showed an organelle-like body with orange fluorescence indicative of phycobilins. Using this fluorescence signal, cells were sorted by flow cytometry and probed. Hybridized cells contained a 4',6'-diamidino-2-phenylindole-stained organelle resembling a plastid with a nucleomorph. This suggests that they may be secondary endosymbiotic algae. Pending the isolation of living cells and their formal description, these algae have been termed picobiliphytes.
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