期刊
DEVELOPMENT
卷 134, 期 2, 页码 273-284出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.02653
关键词
axon guidance; midline; repulsion; roundabout; slit; Spectrin; Ankyrin; Drosophila
资金
- NIGMS NIH HHS [GM 49301] Funding Source: Medline
- NINDS NIH HHS [NS046333, R01 NS046333] Funding Source: Medline
alpha- and beta-Spectrin are major components of a submembrane cytoskeletal network connecting actin filaments to integral plasma membrane proteins. Besides its structural role in red blood cells, the Spectrin network is thought to function in non-erythroid cells during protein targeting and membrane domain formation. Here, we demonstrate that beta-Spectrin is required in neurons for proper midline axon guidance in the Drosophila embryonic CNS. In beta-spectrin mutants many axons inappropriately cross the CNS midline, suggesting a role for beta-Spectrin in midline repulsion. Surprisingly, neither the Ankyrin-binding nor the pleckstrin homology ( PH) domains of beta- Spectrin are required for accurate guidance decisions. beta-Spectrin is dependent upon beta-Spectrin for its normal subcellular localization and/or maintenance, whereas beta-spectrin mutants exhibit a redistribution of beta-Spectrin to the axon scaffold. beta-spectrin mutants show specific dose- dependent genetic interactions with the midline repellent slit and its neuronal receptor roundabout (robo), but not with other guidance molecules. The results suggest that beta-Spectrin contributes to midline repulsion through the regulation of Slit-Robo pathway components. We propose that the Spectrin network is playing a role independently of Ankyrin in the establishment and/or maintenance of specialized membrane domains containing guidance molecules that ensure the fidelity of axon repulsion at the midline.
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