期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 263, 期 1-2, 页码 134-141出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2006.09.009
关键词
proteasome; protein turnover; MKP-1; p42/44 MAPK; norepinephrine; rat pineal
We have previously shown that mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1) is induced at night under the control of a photoneural system in the rat pineal gland. Because of the established roles of MAPKs, glucocorticoids and proteasome activity in regulating MKP-1 expression in other cell types, their relative contributions to MKP-1 regulation were investigated in rat pinealocytes. We found that neither inhibition of MAPKs nor treatment with dexamethasone affected norepinephrine-stimulated MKP-1 expression. In contrast, treatment with proteasome inhibitors increased norepinephrine-stimulated MKP-1 protein levels and abolished the decline in norepinephrine-stimulated MKP-1 protein levels caused by inhibition of transcription or translation, or blockade of (x-adrenergic receptors. Taken together, our results indicate that in rat pinealocytes, the continuous and rapid turnover of MKP-1 protein allows for its rapid induction but is not sufficient to generate the sustained increase in MKP-1 expression post-adrenergic stimulation. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
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