期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 17, 期 2, 页码 456-460出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2006.10.020
关键词
glycoconjugate; DNA polymerase; modified nucleotide
Novel deoxyribonucleotide triphosphates bearing maltose or lactose groups were synthesized as substrates for DNA polymerase. The incorporation efficiencies of these modified substrates were investigated in both primer extension reactions and PCR. The stability and conformation of saccharide-modified dsDNAs were assessed by UV absorbance melting experiments and CD analysis. Enzymatic incorporation of saccharide-modified substrates can be used for the efficient production of saccharide-modified DNAs. (c) 2006 Elsevier Ltd. All rights reserved.
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