期刊
NEUROSCIENCE LETTERS
卷 411, 期 3, 页码 200-205出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2006.09.093
关键词
peripheral benzodiazepine receptor; neuroinflammation; small animal PET; cerebral ischemia; activated microglia
资金
- Intramural NIH HHS Funding Source: Medline
Peripheral benzodiazepine, receptors (PBRs) are upregulated on activated microglia. We recently developed a promising positron emission tomography (PET) ligand, [C-11]PBR28, with high affinity and excellent ratio of specific to nonspecific binding. We assessed the ability of [C-11]PBR28 PET to localize PBRs in a rat permanent middle cerebral artery occlusion (MCAO) model of neuroinflammation. [C-11]PBR28 was intravenously administered to rats at 4 and 7 days after permanent MCAO. In all experiments, arterial blood was sampled for compartmental modeling of regional distribution volumes, and rat brains were sampled after imaging for in vitro [H-3]PK11195 autoradiography and histological evaluation. [C-11]PBR28 PET and [H-3]PK 11195 autoradiography showed similar areas of increased PBRs, especially in the peri-ischemic core. Results from these in vivo and in vitro methods were strongly correlated. In this first study to demonstrate neuroinflammation in vivo with small animal PET, [C-11]PBR28 had adequate sensitivity to localize and quantify the associated increase in PBRs. Published by Elsevier Ireland Ltd.
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