期刊
NEURON
卷 53, 期 2, 页码 217-232出版社
CELL PRESS
DOI: 10.1016/j.neuron.2006.12.012
关键词
-
资金
- NEI NIH HHS [R01 EY013613] Funding Source: Medline
- NHLBI NIH HHS [HL81012, P50 HL081012] Funding Source: Medline
- NICHD NIH HHS [P30 HD018655-25, P30 HD018655, HD18655] Funding Source: Medline
- NINDS NIH HHS [NS45500, R01 NS045500, R01 NS045500-20] Funding Source: Medline
We report the results of a genetic screen to identify molecules important for synapse formation and/or maintenance. siRNAs were used to decrease the expression of candidate genes in neurons, and synapse development was assessed. We surveyed 22 cadherin family members and demonstrated distinct roles for cadherin-11 and cadherin-13 in synapse development. Our screen also revealed roles for the class 4 Semaphorins Sema4B and Sema4D in the development of glutamatergic and/or GABAergic synapses. We found that Sema4D affects the formation of GABAergic, but not glutamatergic, synapses. Our screen also identified the activity-regulated small GTPase Rem2 as a regulator of synapse development. A known calcium channel modulator, Rem2 may function as part of a homeostatic mechanism that controls synapse number. These experiments establish the feasibility of RNAi screens to characterize the mechanisms that control mammalian neuronal development and to identify components of the genetic program that regulate synapse formation and/or maintenance.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据