期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 352, 期 3, 页码 662-667出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2006.11.065
关键词
Na+/K+ ATPase; dexamethasone; SGK1; insulin secreting cells
Glucocorticoids blunt insulin release, an effect partially due to activation of Kv channels. Similar to those channels Na+/K+ ATPase activity repolarizes the plasma membrane. The present study explored whether glucocorticoids increase the Na+/K+ ATPase activity in pancreatic beta-cells. The glucocorticoid dexamethasone (100 nmol/l for I day) significantly increased Na+/K+ ATPase alpha(1)/beta(1)-subunit transcript levels and ouabain-sensitive outward current reflecting Na+/K+ ATPase activity in INS-I cells, effects blunted by glucocorticoid-receptor-blocker RU487 (1 mu mol/l). Dexamethasone (100 nmol/l) increased K+ current in beta-cells from wild type mice but not from knockout mice lacking functional serum and glucocorticoid inducible kinase SGK1. Thus, glucocorticoids indeed up-regulate Na+/K+ ATPase activity, an effect requiring SGK1. (c) 2006 Elsevier Inc. All rights reserved.
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