期刊
NEUROREPORT
卷 18, 期 2, 页码 153-157出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/WNR.0b013e328010d4fa
关键词
dorsal root ganglia; nerve injury; neuropathic pain; neurite outgrowth; osteopontin
资金
- MRC [G0300028] Funding Source: UKRI
- Medical Research Council [G0300028] Funding Source: researchfish
- Medical Research Council [G0300028, G0300028(65120)] Funding Source: Medline
Osteopontin expression has previously been demonstrated in the adult rat dorsal root ganglion, although its function remains unclear. Here, we demonstrate, using real-time reverse transcription-polymerase (RT-PCR) chain reaction, that osteopontin mRNA expression is increased I and 3 weeks following sciatic nerve section (axotomy). Further, immunohistochemical staining suggests that this increase is restricted to neurons already expressing the protein. Osteopontin knock-out animals have significantly increased mechanosensory thresholds in the intact adult compared with the wild-type controls; however no differences in allodynia are noted between genotypes using a model of neuropathic pain. Lastly, exogenous recombinant osteopontin has no effect on neurite outgrowth from adult wild-type sensory neurons, nor were differences in neurite outgrowth observed in osteopontin knock-out animals compared with wild-type controls.
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