期刊
FEBS LETTERS
卷 581, 期 2, 页码 309-314出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2006.12.033
关键词
tRNA; transamidation; Asn-tRNA; Gln-tRNA; evolution
资金
- NIGMS NIH HHS [GM22854, R37 GM022854, R01 GM022854] Funding Source: Medline
The important identity elements in tRNA(Gln) and tRNA(Asn) for bacterial GatCAB and in tRNA(Gln) for archaeal GatDE are the D-loop and the first base pair of the acceptor stem. Here we show that Methanothermobacter thermautotrophicus GatCAB, the archaeal enzyme, is different as it discriminates Asp-tRNA(Asp) and Asp-tRNA(Asn) by use of U49, the D-loop and to a lesser extent the variable loop. Since archaea possess the tRNA(Gln)-specific amidotransferase GatDE, the archaeal GatCAB enzyme evolved to recognize different elements in tRNA(Asn) than those recognized by GatDE or by the bacterial GatCAB enzyme in their tRNA substrates. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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