期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1140, 期 1-2, 页码 140-146出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2006.11.079
关键词
nanoHPLC; monolithic columns; column technology; capillary; mass spectrometry; proteins; repeatability; reproducibility; validation
资金
- NIGMS NIH HHS [R01 GM048364-16, R01 GM044885, GM44885] Funding Source: Medline
Monolithic poly(butyl methacrylate-co-ethylene dimethacrylate) capillary columns have been prepared via either thermally or photochemically initiated polymerization of the corresponding monomers and the repeatability of their preparation has been explored. Three separate batches of 5 columns each were prepared using thermal and photochemical initiation for a total of 30 columns. All 30 capillary columns were tested in liquid chromatography-electrospray ionisation mass spectrometry mode for the separation of a model mixture of three proteins-ribonuclease A, cytochrome c and myoglobin. Excellent repeatability of retention times was observed for the proteins as evidenced by relative standard deviation (RSD) values of less than 1.5%. Somewhat broader variations with RSD values of up to 10% were observed for the pressure drop in the columns. The stability of retention times was also monitored using a single monolithic column and no significant shifts in either retention times or back pressure was observed in a series of almost 2200 consecutive protein separations. (c) 2006 Elsevier B.V. All rights reserved.
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