期刊
JOURNAL OF NEUROSCIENCE METHODS
卷 159, 期 2, 页码 268-276出版社
ELSEVIER
DOI: 10.1016/j.jneumeth.2006.07.024
关键词
in vivo imaging; neuronal tracing; callosal connections; VI connections
资金
- NEI NIH HHS [R01-EY-05935, R01-EY-016184] Funding Source: Medline
From the moment the mouse model took center stage for studies of cortical arealization and map formation, there was an urgent need for methods to identify areal borders in the living animal. The need was met in part by intrinsic optical signal imaging, which has been successfully applied to map topographic representations in primary visual, auditory and somatosensory cortex. However, the challenge remains to register these maps to the underlying structure. This is especially important for studies of the mouse brain in which cortical areas are often only a few hundred microns across. Here, we show that in visual cortex neuronal tracing with fluororuby and fluoroemerald can be used for transcranial imaging through the intact skull of callosal connections from the opposite side of the brain, and for mapping of topographic striate-extrastriate cortical pathways in living mice. Because callosal connections are important landmarks for cortical areas, the new method will allow registration of functional maps to underlying structures and facilitate targeted single-unit recordings in identified cortical areas. (c) 2006 Elsevier B.V. All rights reserved.
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