期刊
JOURNAL OF BIOTECHNOLOGY
卷 128, 期 1, 页码 50-60出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2006.09.021
关键词
gene expression; ferritin heavy chain; Chinese hamster ovary cell; stationary phase
An expression vector has been generated using a gene highly expressed under conditions found in atypical fed-batch bioreactor process. The ferritin heavy chain (HC) gene exhibits higher levels of expression in the late stages of a fed-batch bioreactor than in the early stages. This property was considered advantageous for an expression vector, since the maximal cell density would coincide with maximal expression. The rat ferritin HC genomic region was isolated and converted into an expression vector where large segments of 5' and 3' flanking regions were included in an attempt to recreate the same high level of expression in stably transfected cells. Expression from the resulting ferritin HC vector was compared to vectors containing the commonly used strong promoters, CMV IE, and SV40 early promoter/enhancer, in the generation of stable transfectants. The ferritin HC vector was able to generate cell lines with significantly higher expression levels than those under the control of the viral promoters. (c) 2006 Elsevier B.V. All rights reserved.
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