期刊
JOURNAL OF BIOTECHNOLOGY
卷 128, 期 1, 页码 32-40出版社
ELSEVIER
DOI: 10.1016/j.jbiotec.2006.09.007
关键词
hepatitis B virus RNA interference; retrovirus vector; HepG2.2.15 cells
RNA interference (RNAi) of virus-specific genes has emerged as a potential antiviral strategy. In order to suppress hepatitis B virus (HBV) expression and replication, a retrovirus-based RNAi system was developed, which utilized the U6-RNA polymerase III (Pol III) promoter to drive efficient expression and deliver the HBV-specific short hairpin RNAs (shRNAs) in HepG2.2.15 (2215) cells. In this system, the retrovirus vector with a puromycin selection marker was integrated into the host cell genome and allowed stable expression of shRNAs. In Puro-resistant 2215 cells, the levels of both HBV protein and mRNA were dramatically reduced by over 88% and HBV replication was suppressed. The results demonstrated that retrovirus-based RNAi technology will have foreseeable applications both in experimental biology and molecular medicine. (c) 2006 Elsevier B.V. All rights reserved.
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